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Image Search Results
Journal: Infection and Immunity
Article Title: YraP Contributes to Cell Envelope Integrity and Virulence of Salmonella enterica Serovar Typhimurium
doi: 10.1128/IAI.00829-17
Figure Lengend Snippet: Adherence and invasion of the S. Typhimurium SL1344 and isogenic yraP mutant to various cell types. (A) Images of J774 murine macrophages infected with the S. Typhimurium SL1344 parental strain and the respective yraP mutant. Samples expressing GFP for 2 h were fixed and stained with ActinRed before visualization with a Nikon AR1 inverted confocal microscope using 540/565-nm and 485/510-nm excitation and emission for ActinRed and GFP, respectively. (B to D) Adherence and invasion of S. Typhimurium SL1344 and yraP mutants to the murine macrophage cell line J774 (B), the human intestinal cell line CaCo2 (C), and bone marrow-derived macrophages from C57BL/6 mice (D). Cells were infected at an MOI of 1:100 and incubated for 2 h before lysing and enumeration of the bacterial CFU (adhesion) or treatment with gentamicin for a further 2 h (invasion). Individual data points represent the means of three technical replicates from a single experiment; the bars represent the means of the three independent experiments. (E) The survival of S. Typhimurium SL1344 and isogenic yraP mutant in the murine macrophage cell line, J774, during a time course of 2 and 24 h postinfection (pi). Individual data points represent the means of three technical replicates from two independent experiments. (F) Adherence and invasion of S. Typhimurium SL1344 and yraP mutant to the murine macrophage cell line, J774, in the presence or absence of IFN-γ. Individual data points represent the means of five technical replicates from two biological repeats. NBS, not biologically significant.
Article Snippet: Samples expressing GFP for 2 h were fixed and stained with ActinRed before visualization with a
Techniques: Mutagenesis, Infection, Expressing, Staining, Microscopy, Derivative Assay, Incubation